Localization of GABAA receptors in the rabbit retina |
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Authors: | U. Greferath U. Grünert F. Müller H. Wässle |
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Affiliation: | (1) Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany;(2) Present address: Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Postfach 1913, D-52428 Jülich, Germany |
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Abstract: | The distribution of gamma-aminobutyric acidA (GABAA) receptors in the rabbit retina is investigated and compared with the distribution of GABAergic neurons using immunocytochemical methods. Antibodies against the 1, 2/3, and 2 subunits of the GABAA receptor label subpopulations of bipolar, amacrine and ganglion cells. Double labeling experiments show that the 2 subunit is colocalized with the 1 and the 2/3 subunits in bipolar, amacrine and ganglion cells. Electron microscopy reveals that in the outer plexiform layer, GABAA receptor immunoreactivity is present on dendrites of cone bipolar cells adjacent to the cone pedicles. Bipolar cell dendrites are also receptor-positive at synapses from interplexiform cells. Some receptor immunoreactivity is found intracellularly in processes of horizontal cells. In the inner plexiform layer, GABAA receptor immunoreactivity is present on both rod bipolar and cone bipolar axon terminals at putative GABAergic input sites. Amacrine and ganglion cell processes in sublamina a and b are also labeled. |
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Keywords: | GABAA receptors Light microscopy Electron microscopy /content/w15t4g6r48v5r174/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >1 subunit /content/w15t4g6r48v5r174/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >2/3 subunit /content/w15t4g6r48v5r174/xxlarge947.gif" alt=" gamma" align=" MIDDLE" BORDER=" 0" >2 subunit Immunocytochemistry Rabbit (New Zealand) |
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