Cloning and expression of the dihydroorotate dehydrogenase from Toxoplasma gondii |
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| Authors: | Sierra Pagan Maria L Zimmermann Barbara H |
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| Affiliation: | Department of Biochemistry, University of Puerto Rico School of Medicine, Medical Sciences Campus, P.O. Box 365067, San Juan 00936-5067, Puerto Rico. |
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| Abstract: | A full-length dihydroorotate dehydrogenase (DHODase) sequence was cloned from a Toxoplasma gondii tachyzoite cDNA library. The sequence was most similar to family 2 DHODases, and had a calculated molecular mass of 65.1 kDa. The full-length and two N-terminally truncated T. gondii DHODase sequences were expressed as recombinant proteins. One of the truncated sequences complemented a DHODase-deficient bacterial host. |
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