A rapid method for the determination of the base composition of bacterial DNA |
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| Authors: | Josef Flossdorf |
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| Affiliation: | Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig F.R.G. |
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| Abstract: | A rapid method to evaluate the (G+C)-content of bacterial DNA is described. About 2 × 108 cells are lysed by the combined action of detergents and enzymes and put into a CsCl-gradient without any purification. Up to five samples may be run at once. The high background absorption of cell-derived material other than DNA is overcome by novel collimating optics. |
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