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A simple assay of cyclic-AMP phosphodiesterase in the presence of interfering enzymes in Vibrio harveyi |
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| Authors: | John C Makemson |
| Institution: | Department of Biological Sciences, Florida International University, Miami, FL 33199 U.S.A. |
| Abstract: | PEI-cellulose thin layer chromatography is used to separate the reaction products formed from cyclic-AMP initiated by phosphodiesterase in crude extracts of bacteria. |
| Keywords: | Vibrio harveyi PDE cyclic-AMP phosphodiesterase c-AMP adenosine cyclic monophosphate ADE adenosine ATP ADP and AMP the tri- di- and monophosphates of adenosine respectively PEI polyethyleneimine TCA trichloroacetic acid ADN adnine HX hypoxanthine INO inosine |
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