Modifications enhancing reproducibility and sensitivity in the turbidimetric assay of hyaluronidase |
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| Authors: | Y.-C. Tam E.C.S. Chan |
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| Affiliation: | Department of Microbiology and Immunology and Department of Oral Biology, McGill University, Montreal, PQ H3A 2B4, Canada |
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| Abstract: | Modifications in the conditions for substrate precipitation and wavelength of measurement enhanced the accurcy and sensitivity of detecting the residual hyaluronic acid in the turbidimetric assay of hyaluronidase. These modifications also eliminated the need to used stabilized plasma for substrate coprecipitation and the variability of turbidity readings resulting from small inconsistencies in the timing ofturbidity development. The modified technique was used to determine hyaluronidase activity reproducibly in complex spent media from bacterial cultures without any interference from extraneous substances. |
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| Keywords: | To whom correspondence should be addressed. |
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