枣树离体叶片不定芽再生体系建立的研究

建立了木枣无菌试管苗快繁体系,以无菌苗叶片为外植体,对影响离体叶片不定芽直接再生的因素进行了研究.试验结果表明,TDZ比BA能更有效地诱导叶片不定芽的再生;褐化是抑制不定芽再生频率提高的关键因子,培养基中添加PVP、V c及改变生长素的种类和浓度均不能促进不定芽再生;添加A gNO3能够减轻褐化并可以大幅度提高再生频率,同时培养初期经过3周避光培养更有利于提高再生效率.因此,以附加2.0 m g/L TDZ和0.2 m g/L IBA的M S培养基,并添加5.0 m g/L A gNO3,可以高效诱导木枣离体叶片不定芽再生,再生频率最高达98.3%.不定芽在附加0.2 m g/L IBA和0.5 m g/L GA3的M S培养基上进行继代伸长培养,当不定芽长至3 cm时,转接至附加0.4 m g/L IBA的1/2 M S培养基上可以良好地诱导生根.

In Vitro Plant Regeneration with Adventitious Buds of Zizyphus jujuba Leaves

The study established a system of rapid Zizyphus jujuba micro-propagation and explored the factors affecting the direct in vitro regeneration with adventitious buds of Z.jujuba leaves.The result indicted that TDZ more effectively induced the leaves to produce adventitious buds than BA,browning was the key factor to constrain the regeneration frequency with adventitious buds;the PVP and Vc additions to the medium and the changes of the hormone kinds and concentrations in the medium could not help the adventitious buds occur;AgNO_3 addition could reduce browning and thus greatly increased the regeneration frequency and the lightless culture in the first three week was much more conducive to increasing the regeneration frequency.Therefore,addition of 2.0 mg/L TDZ and 0.2 mg/L IBA as well as 5.0 mg/L AgNO_3 to the MS medium could highly effectively induced Z.jujuba leaves to grow adventitious buds,so that the regeneration frequency reached as high as 98.3%.The adventitious buds were successively cultured in the MS mediums to which 0.2 mg/L IBA and 0.5 mg/L GA_3 were added until they grew 3 cm long roots and then they were transferred and cultured in the 1/2 MS medium to which 0.4 mg/L IBA was added,and this could better induce them to root.