<Emphasis Type="Italic">Deinococcus petrolearius</Emphasis> sp. nov. isolated from crude oil recovery water in China |
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Authors: | Lijun Xi Nenghu Qiao Jingjing Zhang Jing Li Dejian Liu Jing You Jianguo Liu |
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Institution: | 1.State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology,China University of Petroleum,Qingdao,China;2.Petroleum Production Engineering Institute of Huabei Oilfield Ltd,Renqiu,China;3.School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,China |
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Abstract: | A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1T, was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m?2). Based on 16S rRNA gene sequence comparisons, strain 6.1T shows high similarity to Deinococcus citri DSM 24791T (98.1%) and Deinococcus gobiensis I-0T (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH), followed by iso-C17:1 ω9c and C16:0. The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA–DNA hybridization between strain 6.1T and D. citri DSM 24791T was 45.6 ± 7.1% and with D. gobiensis I-OT was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1T represents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1T (= CGMCC 1.15053T = KCTC 33744T). |
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