High-fat-diet impaired mitochondrial function of cumulus cells but improved the efficiency of parthenogenetic embryonic quality in mice |
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| Authors: | Jingjing Li Shuang Wang Bo Wang Hao Wei Xin Liu Jun Hao |
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| Affiliation: | 1. College of Life Sciences, Northwest University, Xi’an, People’s Republic of China;2. Department of Experimental Surgery of Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of China |
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| Abstract: | Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p?p?P450 arom were higher in the treated group than the control group (p?p?
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| Keywords: | Embryo high-fat diet mouse oocyte parthenogenesis |
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