Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">lycopersici</Emphasis> |
| |
Authors: | Carlos A Araiza-Cervantes Víctor Meza-Carmen Guadalupe Martínez-Cadena M Isabel G Roncero Georgina E Reyna-López Bernardo Franco |
| |
Institution: | 1.División de Ciencias Naturales y Exactas, Departamento de Biología,Universidad de Guanajuato,Guanajuato,Mexico;2.Instituto de Investigaciones Químico-Biológicas,Universidad Michoacana de San Nicolás Hidalgo,Morelia,Mexico;3.Departamento de Genética,Universidad de Córdoba,Córdoba,Spain |
| |
Abstract: | The genome sequence of the plant pathogen Fusarium oxysporum f. sp. lycopersici contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in Escherichia coli. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of F. oxysporum f. sp. lycopersici showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of F. oxysporum f. sp. lycopersici is involved in DNA repair and does not act in pathogenicity as an effector. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|