Effect of lexA and ssb genes, present on a uvrA recombinant plasmid, on the UV survival of Escherichia coli K-12 |
| |
Authors: | J A Brandsma J Stoorvogel C A van Sluis P van de Putte |
| |
Institution: | Laboratory of Molecular Genetics, State University of Leiden, Wassenaarseweg 64, 2333 AL LeidenThe Netherlands |
| |
Abstract: | The recombinant plasmid pJA01 contains, besides the uvrA gene, the genes lexA, ubiA and ssb. This plasmid does not fully complement a uvrA mutation in a Rec+ background. Plasmids which contain the uvrA and ssg genes, but not the lexA gene, show a higher but still only partial complementation. Full complementation achieved when the ssb gene us inactivated by insertion of Tn5. Furthermore, it appears that the presence of the ssb gene on a multicopy plasmid sensitizes wild-type cells to UV light. The effect of Ssb (single-strand DNA binding protein) overproduction on UV survival is discussed. |
| |
Keywords: | Cloning excision repair single-strand DNA-binding protein Tn5 transposon maxicells kb kilobase pairs ts thermosensitive U units UV ultraviolet |
本文献已被 ScienceDirect 等数据库收录! |
|