| 猴头菌子实体超临界流体技术萃取活性物质初探 |
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| 引用本文： | 贾薇,余冬生,徐宾,张赫男,贾继宁,杨焱,于荣利,汪雯翰,张劲松. 猴头菌子实体超临界流体技术萃取活性物质初探[J]. 菌物学报, 2018, 37(12): 1780-1791. DOI: 10.13346/j.mycosystema.180178 |
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| 作者姓名： | 贾薇 余冬生 徐宾 张赫男 贾继宁 杨焱 于荣利 汪雯翰 张劲松 |
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| 作者单位： | 1 上海市农业科学院食用菌研究所 上海市农业遗传育种重点开放实验室 农业部南方食用菌资源利用重点实验室 国家食用菌加工技术研发分中心 国家食用菌工程技术研究中心 上海 2014032 浙江工业大学化学工程学院 浙江 杭州 310014 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科创字（2018）第1-1号](G20180101) |
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| 摘 要： | 以猴头菌子实体为原料,萃取物得率作为指标,采用CO2超临界流体萃取技术,以萃取压力和CO2流量等参数为考察因素,结合正交试验获得优化的萃取工艺：萃取压力为30MPa,温度为45℃,时间为1.5h,CO2流量为20g/min,夹带剂（乙醇）与猴头菌子实体的物料比为5:1（mL:g）,在此条件下,萃取物得率为2.78%。与猴头菌子实体的醇提物相比,猴头菌子实体超临界萃取物具有更好的体外抗氧化能力和抗肿瘤活性,本研究结果为合理地开发和利用猴头菌子实体超临界萃取物产品提供科学的数据。
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| 关 键 词： | 猴头菌 CO2超临界流体萃取技术 抗氧化活性 抗肿瘤活性 |
| 收稿时间： | 2018-07-16 |
A preliminary study on extraction of active substance from Hericium erinaceus fruiting body using CO2 supercritical fluid technology |
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| JIA Wei,YU Dong-Sheng,XU Bin,ZHANG He-Nan,JIA Ji-Ning,YANG Yan,YU Yong-Li,WANG Wen-Han,ZHANG Jing-Song. A preliminary study on extraction of active substance from Hericium erinaceus fruiting body using CO2 supercritical fluid technology[J]. Mycosystema, 2018, 37(12): 1780-1791. DOI: 10.13346/j.mycosystema.180178 |
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| Authors: | JIA Wei YU Dong-Sheng XU Bin ZHANG He-Nan JIA Ji-Ning YANG Yan YU Yong-Li WANG Wen-Han ZHANG Jing-Song |
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| Affiliation: | 1 National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China2 College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China |
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| Abstract: | Active substance from fruiting body of Hericium erinaceus was extracted using supercritical CO2 fluid technology, and the effects of the extraction pressure and CO2 flow rate in process of extraction and separation on extract yields were investigated by orthogonal experiment. The optimal conditions were extraction pressure of 30MPa at 45°C, extraction duration of 1.5h, and carbon dioxide flow rate of 20g/min. The ratio of entrainer (ethanol) to H. erinaceus fruiting body weight was 5:1 (mL:g). Under these conditions, crude extract yield was 2.78%. The supercritical fluid CO2 extracts of H. erinaceus fruiting bodies showed better antioxidant capability in vitro and anti-tumor activity as compared with ethanol extraction. These results provide fundamental data for exploiting functional products of the fungus. |
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| Keywords: | Hericium erinaceus CO2 supercritical fluid extraction technology antioxidant activity anti-tumor activity |
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