| 基于香草醛-高氯酸显色反应测定灵芝三萜的方法探讨与修正 |
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| 引用本文： | 张倩倩,黄青.基于香草醛-高氯酸显色反应测定灵芝三萜的方法探讨与修正[J].菌物学报,2018,37(12):1792-1801. |
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| 作者姓名： | 张倩倩 黄青 |
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| 作者单位： | 1 中国科学院合肥物质科学研究院 技术生物与农业工程研究所 安徽 合肥 2300312 中国科学技术大学 安徽 合肥 230026 |
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| 基金项目： | 国家自然科学基金(11635013);国家自然科学基金(11775272);国家自然科学基金(11475217) |
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| 摘 要： | 本文报道了基于香草醛-高氯酸显色反应的分光光度法定量测定灵芝三萜的修正方法,并对该方法应用进行了探讨和优化。采用此方法检测了灵芝子实体中含量较高的几种三萜酸,结果表明若采用齐墩果酸为标准品检测灵芝三萜,检测结果远低于真实值。在光谱分析上,研究表明对紫外-可见光扫描吸收峰进行面积积分,获得的标准曲线的线性关系更优。
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| 关 键 词： | 灵芝 分光光度法 灵芝三萜酸 |
| 收稿时间： | 2018-08-09 |
Revised method for determining Ganoderma lingzhi terpenoids by UV-Vis spectrophotometry based on colorimetric vanillin perchloric acid reaction |
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| Authors: | ZHANG Qian-Qian HUANG Qing |
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| Institution: | 1 Institute of Technical Biology & Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China2 University of Science and Technology of China, Hefei, Anhui 230026, China |
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| Abstract: | This paper reports the revised method of determining the Ganoderma triterpenoids by UV-Vis absorption spectroscopy based on the colorimetric reaction between triterpenoid with perchloric acid and vanillin, from this method the standard curves are derived and optimized. Several main triterpenoids purified from Ganoderma lingzhi fruiting body were analyzed, and our results showed that if coleanolic acid was used as standard to evaluate the triterpenoids, the detection results were far below the true values. Spectral analysis was proved that better linear relationship of the standard curve could be obtained by integrating the area of the UV-visible absorption bands. |
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| Keywords: | Ganoderma lingzhi spectrophotometry terpenoids |
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