刺芹侧耳菌丝体固体发酵多糖在斑马鱼模型上的降血脂活性

高脂血症是诱导脂肪肝、高血压、动脉粥样硬化和心脑血管疾病的一个关键风险因素。本课题组前期研究已经在细胞水平、小鼠和斑马鱼动物模型上证实,刺芹侧耳多糖具有抑制体内脂质积累的生物活性。本文利用农林废弃物玉米芯和麦麸作为主要培养原料,通过固体发酵获得刺芹侧耳菌丝体多糖PESF（polysaccharide from Pleurotus eryngii mycelium solid-state fermentation）;进而,采用斑马鱼幼鱼和成鱼高脂动物模型,研究了刺芹侧耳菌丝体多糖在动物体内的降脂效率并解析了可能的降血脂途径和机理。实验结果证实,剂量400μg/mL的PESF不仅可显著抑制高脂饮食引起的斑马鱼幼鱼体内脂质积累,而且也可以有效抑制高脂饮食下斑马鱼成鱼的肝脏和肠道组织内的脂质积累,证实刺芹侧耳多糖具有显著抑制动物体内脂质积累的活性。这些结果建议,刺芹侧耳多糖降血脂的途径可能是通过降低肠道对脂类物质的吸收,从而减少了脂滴在肝脏中的积累。因此,本研究建议刺芹侧耳多糖具有开发成为降脂食品添加剂或者降脂药物原料的潜力。

The lipid-lowering effect of Pleurotus eryngii mycelium polysaccharides in zebrafish models

Hyperlipidemia, a most common form of dyslipidemia with abnormally elevated lipid or lipoprotein levels in the blood, has been regarded as one of major risk factors for cardiovascular disease. Previous studies have demonstrated that polysaccharides from Pleurotus eryngii (king oyster mushroom) are capable of inhibiting lipid accumulation in the cells, in the mice and zebrafish animal models. In this study, polysaccharides were obtained from Pleurotus eryngii mycelium by solid-state fermentation using lignocellulosic waste, corn-cob and wheat bran as substrate. Lipid-lowering efficiency and mechanism of the polysaccharides were detected in zebrafish larva and adult models. It was indicated that polysaccharides obtained from Pleurotus eryngii mycelium solid-state fermentation (PESF) had in vivo activity of reducing lipid accumulation in the body of zebrafish larvae fed with high fat diet (HFD), and significantly decreasing accumulation of lipid in livers through blocking intestinal lipid attachment and absorption in both zebrafish larva and adult models. Our findings suggest that PESF can be used as a valuable lipid-lowering food additive or raw materials for producing lipid-lowering drugs for hypolipidemic and hepatoprotective treatments.