大革耳子实体多糖抗氧化活性

作者对大革耳子实体多糖的抗氧化能力及单糖组分进行了分析,并探究了大革耳子实体多糖体外对羟自由基、超氧阴离子自由基、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基、2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸[2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid),ABTS]自由基的清除能力和铁离子还原能力;以人正常肝细胞系LO2为材料建立了过氧化氢细胞氧化损伤模型,并探讨大革耳子实体多糖在细胞水平的抗氧化能力;通过苯酚硫酸法及HPLC检测了子实体多糖的单糖含量及组分。体外化学抗氧化实验结果显示,大革耳子实体多糖对羟自由基、超氧阴离子、DPPH自由基和ABTS自由基的清除能力较强,且具有较高的铁离子还原能力;细胞水平抗氧化实验表明,大革耳子实体多糖对人正常肝细胞系LO2的H₂O₂氧化损伤具有显著的保护作用,并能极显著提高受损细胞内过氧化氢酶（catalase,CAT）（P<0.01）及超氧化物歧化酶（superoxide dismutase,SOD）（P<0.01）的活力。大革耳子实体活性多糖主要单糖含量及组分依次为：葡萄糖（2 985.50mg/kg）、甘露糖（1 867.23mg/kg）、木糖（814.98mg/kg）、半乳糖（724.24mg/kg）、岩藻糖（443.72mg/kg）、葡萄糖醛酸（419.41mg/kg）、鼠李糖（81.18mg/kg）、阿拉伯糖（64.40mg/kg）、核糖（39.95mg/kg）、半乳糖醛酸（24.40mg/kg）。本研究结果为更好的推广应用和科学开发大革耳提供了基础资料。

Polysaccharide antioxidant activities of Panus giganteus

The antioxidative capacity and monosaccharide composition of the polysaccharide from the fruiting body of Panus giganteus were analyzed. The scavenging ability in vitro to hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl, DPPH free radical, superoxide anion radical, 2,2?-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid, ABTS free radical and iron ion reducing ability were explored. Human liver cell line LO2 was used as a material to establish a model of hydrogen peroxide cell oxidative damage, and the anti-oxidation ability of fruiting bodies of Panus giganteus at the cellular level was explored. The monosaccharide content and composition of the polysaccharide in the fruiting bodies were also detected by phenol sulfuric acid method and HPLC. The results of antioxidant activity test showed that the polysaccharides of Panus giganteus had strong scavenging ability to hydroxyl radicals, superoxide anions, DPPH free radicals and ABTS free radicals, and also had high iron ion reducing ability. The results of intracellular antioxidant activity assay indicated that Panus giganteus polysaccharide had protective effect to oxidize H₂O₂ in hepatocyte LO2, and could significantly increase the activity of catalase (CAT) (P<0.01) and superoxide dismutase (SOD) in the damaged cells at 10mg/mL (P<0.01); The main monosaccharide content of the active polysaccharides in the fruiting body of Panus giganteus included glucose (2 985.50mg/kg), mannose (1 867.23mg/kg), xylose (814.98mg/kg), galactose (724.24mg/kg), and fucose (443.72mg/kg), glucose aldehyde (419.41mg/kg), L-rhamnose monohydrate (81.18mg/kg), L-arabinose (64.40mg/kg), ribose (39.95mg/kg), and galactose aldehyde (24.40mg/kg). The results of this study provide basic data for the industrial popularization and commercial development of Panus giganteus.