Detection of Sclerotinia sclerotiorum in Planta by a Real-time PCR Assay |
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| Authors: | Yanni Yin Laisong Ding Xin Liu Jinghui Yang Zhonghua Ma |
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| Affiliation: | Authors' addresses: Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029;;Zhenjiang Institute of Agricultural Science, 112 Ninghang Road, Jurong, China (correspondence to Z. Ma. E-mail: ) |
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| Abstract: | Stem rot caused by Sclerotinia sclerotiorum is a very serious disease on oilseed rape worldwide. In this study, a pair of polymerase chain reaction (PCR) primers was designed based on the nucleotide sequence of a DNA region amplified by a microsatellite primer M13. The primer pair amplified a 252-bp fragment from all S. sclerotiorum isolates collected from oilseed rapes at different locations in different years, but not from any other fungus tested. Using this pair of primers, a real-time PCR assay was developed to rapidly detect early infection of S. sclerotiorum on petals of oilseed rape. The real-time PCR assay developed in this study could help growers make a timely decision on fungicide application. |
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| Keywords: | oilseed rape real-time polymerase chain reaction Sclerotinia sclerotiorum |
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