Regulation of R100 conjugation requires traM in cis to traJ |
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Authors: | Walter B. Dempsey |
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Affiliation: | Veterans' Affairs Medical Center and University of Texas Southwestern Medical Center, Dallas, Texas 75216, USA. |
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Abstract: | Deletion mutants of R100-1 were constructed by classical methods to remove various segments of the traM open reading frame, pTraM-binding sites and the traM promoters. Complementation tests showed that traM was efficiently complemented only when the frans-acting fragment contained both the complete traM gene and the adjacent traJ promoter and leader sequences. The conclusion is that traM and traJ constitute a complex operon. A deletion mutant lacking all of the fraJ gene, and one containing a frameshifting traM deletion, retained the ability to transfer at a low level, thereby showing that neither pTraM nor pTraJ is absolutely essential for transfer. |
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