Fluorescence-based assay to measure the real-time kinetics of nucleotide incorporation during transcription elongation |
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Authors: | Tang Guo-Qing Anand Vasanti S Patel Smita S |
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Affiliation: | Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 683 Hoes Lane, Piscataway, NJ 08854, USA |
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Abstract: | |
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Keywords: | RNAP, RNA polymerase GTP, guanosine 5&prime -triphosphate 2AP, 2-aminopurine 3&prime -dC, 3&prime -deoxycytidine T, template NT, nontemplate |
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