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Role of the tail in the regulated state of myosin 2
Authors:Jung Hyun Suk  Billington Neil  Thirumurugan Kavitha  Salzameda Bridget  Cremo Christine R  Chalovich Joseph M  Chantler Peter D  Knight Peter J
Institution:
  • 1Institute of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
  • 2Division of Electron Microscopic Research, Korea Basic Science Institute, 52 Eoeun-dong, Daejeon 305-333, Korea
  • 3Structural Biology Lab, CBMR, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT) University, Vellore, 632014, Tamil Nadu, India
  • 4Department of Biochemistry and Molecular Biology, University of Nevada School of Medicine, 1664 North Virginia Street, Reno, NV 89557, USA
  • 5Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858-4354, USA
  • 6Unit of Molecular and Cellular Biology, Royal Veterinary College, University of London, Royal College Street, London, NW1 0TU, UK
  • Abstract:Myosin 2 from vertebrate smooth muscle or non-muscle sources is in equilibrium between compact, inactive monomers and thick filaments under physiological conditions. In the inactive monomer, the two heads pack compactly together, and the long tail is folded into three closely packed segments that are associated chiefly with one of the heads. The molecular basis of the folding of the tail remains unexplained. By using electron microscopy, we show that compact monomers of smooth muscle myosin 2 have the same structure in both the native state and following specific, intramolecular photo-cross-linking between Cys109 of the regulatory light chain (RLC) and segment 3 of the tail. Nonspecific cross-linking between lysine residues of the folded monomer by glutaraldehyde also does not perturb the compact conformation and stabilizes it against unfolding at high ionic strength. Sequence comparisons across phyla and myosin 2 isoforms suggest that the folding of the tail is stabilized by ionic interactions between the positively charged N-terminal sequence of the RLC and a negatively charged region near the start of tail segment 3 and that phosphorylation of the RLC could perturb these interactions. Our results support the view that interactions between the heads and the distal tail perform a critical role in regulating activity of myosin 2 molecules through stabilizing the compact monomer conformation.
    Keywords:RLC  regulatory light chain  ELC  essential light chain  SmM  smooth muscle myosin  ScM  scallop cross-striated adductor muscle myosin  HMM  heavy meromyosin  EM  electron microscopy  SmHMM  smooth muscle heavy meromyosin  EGTA  ethylene glycol bis(β-aminoethyl ether) N  N&prime  -tetraacetic acid
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