Proteomic analysis of ribosomes: translational control of mRNA populations by glycogen synthase GYS1 |
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Authors: | Fuchs Gabriele Diges Camille Kohlstaedt Lori A Wehner Karen A Sarnow Peter |
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Institution: | 1 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA2 Proteomics/Mass Spectrometry Laboratory at the University of California at Berkeley, Berkeley, CA 94720, USA |
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Abstract: | Ribosomes exist as a heterogenous pool of macromolecular complexes composed of ribosomal RNA molecules, ribosomal proteins, and numerous associated “nonribosomal” proteins. To identify nonribosomal proteins that may modulate ribosome activity, we examined the composition of translationally active and inactive ribosomes using a proteomic multidimensional protein identification technology. Notably, the phosphorylated isoform of glycogen synthase, glycogen synthase 1 (GYS1), was preferentially associated with elongating ribosomes. Depletion of GYS1 affected the translation of a subset of cellular mRNAs, some of which encode proteins that modulate protein biosynthesis. These findings argue that GYS1 abundance, by virtue of its ribosomal association, provides a feedback loop between the energy state of the cells and the translation machinery. |
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Keywords: | GYS1 glycogen synthase 1 GSK-3 glycogen synthase kinase 3 EDTA ethylenediaminetetraacetic acid MudPIT multidimensional protein identification technology CIP calf intestinal phosphatase siRNA small inhibitory RNA PBS phosphate-buffered saline HFBA heptafluorobutyric acid HRP horseradish peroxidase TBS Tris-buffered saline |
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