The Structure of Physarum polycephalum hemagglutinin I suggests a minimal carbohydrate recognition domain of legume lectin fold |
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Authors: | Kouno Takahide Watanabe Nobuhisa Sakai Naoki Nakamura Takashi Nabeshima Yuko Morita Masashi Mizuguchi Mineyuki Aizawa Tomoyasu Demura Makoto Imanaka Tsuneo Tanaka Isao Kawano Keiichi |
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Institution: | 1 Department of Structural Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan2 Synchrotron Radiation Research Center, Nagoya University, Nagoya 464-8603, Japan3 Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan4 Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan5 Department of Biological Chemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan6 Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan |
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Abstract: | Physarum polycephalum hemagglutinin I (HA1) is a 104-residue protein that is secreted to extracellular space. The crystal structure of HA1 has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of HA1 lacks a jelly roll motif and is essentially composed of two simple up-and-down β-sheets. This up-and-down β-sheet motif is well conserved in other legume lectin-like proteins derived from animals, plants, bacteria, and viruses. It is more noteworthy that the up-and-down β-sheet motif includes many residues that make contact with the target carbohydrates. Our NMR data demonstrate that HA1 lacking a jelly roll motif also binds to its target glycopeptide. Taken together, these data show that the up-and-down β-sheet motif provides a fundamental scaffold for the binding of legume lectin-like proteins to the target carbohydrates, and the structure of HA1 suggests a minimal carbohydrate recognition domain. |
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Keywords: | CRD carbohydrate recognition domain PFG pulsed-field gradient CBM carbohydrate-binding module DLS dynamic light scattering HSQC heteronuclear single quantum coherence SAD single-wavelength anomalous difference |
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