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Small-angle X-ray scattering study of a Rex family repressor: conformational response to NADH and NAD+ binding in solution
Authors:Wang Ellen  Ikonen Teemu P  Knaapila Matti  Svergun Dmitri  Logan Derek T  von Wachenfeldt Claes
Affiliation:
  • 1 Department of Biochemistry and Structural Biology, Lund University, SE-22100 Lund, Sweden
  • 2 European Molecular Biology Laboratory, Hamburg Outstation, DE-22603 Hamburg, Germany
  • 3 Physics Department, Institute for Energy Technology, NO-2027 Kjeller, Norway
  • 4 MAX-lab, Lund University, SE-22100 Lund, Sweden
  • 5 Department of Biology, Lund University, Sölvegatan 35, SE-22362 Lund, Sweden
  • Abstract:
    Keywords:T-Rex, Thermus aquaticus Rex   B-Rex, Bacillus subtilis Rex   SAXS, small-angle X-ray scattering   DLS, Dynamic light-scattering   NSD, normalized spatial discrepancy   BCA, bicinchoninic acid   NAD(H), nicotinamide-adenine dinucleotide with unspecified oxidation state   FWHM, full width at half maximum   ROP, Rex operator
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