Interaction of Gila monster venom with secretin receptors in rat pancreatic membranes |
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| Authors: | L. Gillet P. Robberecht M. Waelbroeck J.C. Camus P. De Neef W. König J. Christophe |
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| Affiliation: | 1. Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles Boulevard de Waterloo 115, B-1000 Brussels, Belgium;2. Hoechst Aktiengesellschaft, Frankfurt/Main, F.R.G. |
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| Abstract: | The stimulatory effect of Gila monster venom on adenylate cyclase activity in rat pancreatic membranes was compared to that of porcine secretin and porcine VIP. The maximal effect exerted by the venom was identical to that of VIP but significantly lower than that of secretin. The effect of Gila monster venom could, however, be attributed to its interaction with secretin receptors rather than with VIP receptors, at variance with its previously described action on guinea pig pancreatic acini. Adenylate cyclase activation by both Gila monster venom and secretin in rat pancreatic membranes was, indeed: (1) dose-dependently inhibited by two secretin fragments secretin-(4-27) and secretin-(7-27), and (2) more severely depressed than VIP stimulation, after pretreating pancreatic membranes with dithiothreitol (DTT). |
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| Keywords: | Secretin Vasoactive intestinal peptide Gila monster venom Rat pancreatic membranes Adenylate cyclase Dithiothreitol |
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