Enumeration of chlorine-stressed organisms with acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (AOINT) |
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| Authors: | Diane S Herson Balkumar Marthi Mary Anne Payer Dr Katherine H Baker |
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| Institution: | (1) School of Life and Health Sciences, University of Delaware, Newark, Delaware;(2) Department of Biology, Millersville University of Pennsylvania, 17551 Millersville, Pennsylvania, USA |
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| Abstract: | Direct microscopic enumeration ofEnterobacter cloacae with the acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride technique (AOINT) was compared with spread plate counts on nonselective media to establish the usefulness of the former technique in the enumeration of chlorine-stressed cells. Results indicate that the techniques are comparable when the organisms are not stressed. However, AOINT is more sensitive than are plate counts in the detection of chlorine-stressed cells. |
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