| cDNA RDA法克隆小鼠鼻咽部组织特异性表达基因 |
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| 引用本文： | 凌建华 姚开泰. cDNA RDA法克隆小鼠鼻咽部组织特异性表达基因[J]. Acta biochimica et biophysica Sinica, 1998, 30(5): 501-504 |
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| 作者姓名： | 凌建华 姚开泰 |
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| 作者单位： | 湖南医科大学肿瘤研究所 |
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| 摘 要： | 采用ｃＤＮＡ代表性差异分析法（ｃＤＮＡ ＲＤＡ）克隆小鼠鼻咽部组织特异性表达基因。通过三轮消减杂交后，获得了七个差异片段。随机选择两个差异片段ＴＤＰ１和ＴＤＰ４，经ＤＮＡ印迹和ＲＮＡ印迹证实了ＴＤＰ１在鼻咽部特异性表达，而ＴＤＰ４在鼻咽部及食道均有表达，并没有特异性。进一步对这两片段测序分析，并与Ｇｅｎ－Ｂａｎｋ等数据库同源比较，推测ＴＤＰ１和ＴＤＰ４是两个新的基因片段，但是仅ＴＤＰ１在小鼠鼻咽部
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| 关 键 词： | 代表性差异法 克隆 组织特异性表达 基因 |
cDNA RDA Cloning of the Gene Specifically Expressed in Murine Nasopharynx |
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| LING Jian Hua and YAO Kai Tai. cDNA RDA Cloning of the Gene Specifically Expressed in Murine Nasopharynx[J]. Acta biochimica et biophysica Sinica, 1998, 30(5): 501-504 |
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| Authors: | LING Jian Hua and YAO Kai Tai |
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| Affiliation: | LING Jian Hua and YAO Kai Tai * |
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| Abstract: | cDNA representational difference analysis was used to clone genes specifically expressed in murine nasopharynx. 7 difference fragments were obtained after 3 rounds of subtractive hybridization and it was found that TDP1 was specifically expressed in murine nasopharynx, but TDP4 was expressed in murine nasopharynx and esophagus. Comparisons between our sequencing results and data in GenBank show no homology, suggesting that both TDP1 and TDP4 are new genes. |
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| Keywords: | Representational difference analysis clone tissue specific expression gene |
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