High rate of IgE-mediated histamine release from rat mesenteric mast cells. |
| |
Authors: | N Inagaki H Kawasaki H Nagai |
| |
Affiliation: | Department of Pharmacology, Gifu Pharmaceutical University 5-6-1 Mitahorahigashi, Gifu 502-8585, Japan. |
| |
Abstract: | IgE-dependent histamine release from rat mesenteric mast cells was investigated. Excised mesenterium was cut into pieces and incubated with IgE overnight at 4 degrees C for sensitization. Over 10 pieces of mesenterium specimen could be prepared from a rat. Antigen-induced histamine release from mesenterium specimen was initiated rapidly and reached a plateau in 5 min. In an optimal condition, over 50% of total histamine was released. In contrast, unpurified and purified peritoneal mast cells released only 22.5% and 5.3% of total histamine, respectively, upon IgE stimulation. Tranilast, a mast cell stabilizer, inhibited the histamine release from mesenteric mast cells significantly. The mesenterium might be useful material for studying tissue-associated mast cell activation. |
| |
Keywords: | |
|
|