Behavior of phosphatase isoforms during sclerotium formation in Physarum polycephalum |
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Authors: | Tanaka Yuhko Suzuki Yohko Osumi Masako Kaneko Takako S |
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Affiliation: | Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1-Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan. |
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Abstract: | The behavior of phosphatase isoforms under dark-starvation from plasmodium of Physarum polycephalum were investigated to determine their possible roles in sclerotium formation. Two and a half days after dark-starvation, approximately 95% of plasmodia plates formed sclerotia. Specific phosphatase activity increased markedly up to ca. two-fold within the first day of starvation, after which the enzymatic activity decreased rapidly to a level less than the initial level within 2 days of the starvation period. Among the two isoforms of enzyme detected just before sclerotization under dark-starvation conditions, the enzymatic activity of the major isoform (Rm value of 0.6) decreased gradually within 1.5 days of starvation, then linearly to less than 20% of that at the beginning of the observation. Those of other major isoform (Rm value of 0.7) increased up to ca. two-fold within the first day of starvation, then decreased linearly to levels less than that of the first 2 days of the starvation period. Behavior of this isoform strongly suggests that it initiates the formation of sclerotium under dark-starvation conditions. |
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Keywords: | Physarum polycephalum Myxomycetes Slime mould Plasmodia Sclerotia Dark-starvation Phosphatase |
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