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In vitro dissociation-recombination of malate dehydrogenase subunits in Corydalis solida
Authors:A. H. Nagy  Dr. M. O. Siddiqui  Z. G. Kocsis  G. Vida
Affiliation:(1) Department of Genetics, Eötvös Loránd University, Budapest, Hungary;(2) Department of Botany, Patna University, 800005 Patna, India
Abstract:Summary Two allelic forms of NAD specific malate dehydrogenase were found in samples of a wild population of Corydalis solida. The dimeric nature and the origin of the heterodimeric form has been demonstrated by in vitro dissociation and recombination of the subunits detected by subsequent electrophoresis. The method is applicable for polyacrylamide gel electrophoresis of crude leaf extracts of individual MDH isozyme forms.
Keywords:Malate dehydrogenase  Isozyme  Interallelic subunits  Heterodimer  In vitro dissociation  Subunit recombination  Corydalis solida
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