首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Longitudinal Study of Reproductive Performance of Female Cattle Produced by Somatic Cell Nuclear Transfer
Authors:Irina A Polejaeva  Diane M Broek  Shawn C Walker  Wenli Zhou  Mark Walton  Abby D Benninghoff  David C Faber
Institution:1. Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah, United States of America.; 2. ViaGen, L.C., Cedar Park, Texas, United States of America.; 3. Recombinetics, Inc., St. Paul, Minnesota, United States of America.; 4. School of Veterinary Medicine, Utah State University, Logan, Utah, United States of America.; 5. Trans Ova Genetics, L.C., Sioux Center, Iowa, United States of America.; University of Connecticut, United States of America,
Abstract:The objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT) is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo production procedures: (1) superovulation followed by artificial insemination (AI) and embryo collection and (2) ultrasound-guided ovum pick-up followed by in vitro fertilization (OPU-IVF). Collectively, these two studies represent the largest data set available for any species on the reproductive performance of female clones and their genetic donors as measured by their embryo production outcomes in commercial embryo production program. The large-scale study described herein was conducted over a six-year period of time and provides a unique comparison of 96 clones to the 40 corresponding genetic donors. To our knowledge, this is the first longitudinal study on the reproductive performance of cattle clones using OPU-IVF. With nearly 2,000 reproductive procedures performed and more than 9,200 transferable embryos produced, our observations show that the reproductive performance of cattle produced by SCNT is not different compared to their genetic donors for the production of transferable embryos after either AI followed by embryo collection (P = 0.77) or OPU-IVF (P = 0.97). These data are in agreement with previous reports showing that the reproductive capabilities of cloned cattle are equal to that of conventionally produced cattle. In conclusion, results of this longitudinal study once again demonstrate that cloning technology, in combination with superovulation, AI and embryo collection or OPU-IVF, provides a valuable tool for faster dissemination of superior maternal genetics.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号