Effects of Vascular Endothelial Growth Factor 165 on Bone Tissue Engineering |
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| Authors: | Lin Feng Hao Wu Lingling E Dongsheng Wang Fukui Feng Yuwan Dong Hongchen Liu Lili Wang |
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| Institution: | 1. Oral Medical Research Center, Chinese PLA General Hospital, Beijing, P. R. China.; 2. Department of Prosthodontics, Affiliated Stomatological Hospital of LMU, Jinzhou, P. R. China.; University of Oxford, United Kingdom, |
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| Abstract: | To study the relationship between vascular endothelial growth factor (VEGF) and formation and repair of engineering bone, second-generation bone marrow stromal cells (BMSCs) of New Zealand white rabbits that were separated in vitro were transfected with VEGF 165 gene vectors by adenovirus to detect gene expressions. Transfected BMSCs and β-tricalcium phosphate material were complexed and implanted at the femoral injury sites of the study group (n = 12), and the control group (n = 12) were implanted with engineering bones that were not transfected with VEGF. Femoral recoveries of the two groups were observed on the 15th, 30th, 45th and 60th days, and their vascularization and ossification statuses were observed by immunohistochemical methods. The BMSCs transfected with VEGF highly expressed VEGF genes and excreted VEGF. The two groups both experienced increased vascularization and bone volume after implantation (t = 7.92, P<0.05), and the increases of the study group were significantly higher than those of the control group (t = 6.92, P<0.05). VEGF is clinically applicable because it can accelerate the formation and repair of engineering bone by promoting vascularization and ossification. |
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