Self-recognition of N-linked glycans with multivalent GlcNAc, determined as ceramide mimetic conjugate |
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| Authors: | Yoon Seon-Joo Ikeda Shoko Sadilek Martin Hakomori Sen-itiroh Ishida Hideharu Kiso Makoto |
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| Institution: | Division of Biomembrane Research, Pacific Northwest Research Institute, Department of Pathobiology, University of Washington, Seattle, WA 98195, USA. |
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| Abstract: | Aminoceramide mimetic was synthesized and conjugated to N-linked oligosaccharides having multivalent GlcNAc by reductive amination. Ceramide mimetic conjugates with "complex-type" glycan having five or six GlcNAc termini (termed Os Fr. B-Cer) were purified, analyzed by thin-layer chromatography (TLC), and finally characterized by MS/MS analysis through liquid chromatography/mass spectrometry. Binding of Os Fr. B-Cer placed on solid phase polystyrene surface with 3H]cholesterol-labeled liposomes containing Os Fr. B-Cer, or containing various glycosphingolipids (GSLs) was determined. The binding of Os Fr. B-Cer liposomes to Os Fr. B-Cer coated plate was significantly higher than binding of GM3 liposomes. Other GSL liposomes showed no binding. Thus, self-recognition of Os Fr. B-Cer was clearly demonstrated using ceramide mimetic conjugates. |
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| Keywords: | aminoceramide / carbohydrate-to-carbohydrate interaction / glycosphingolipid / homotypic interaction / oligosaccharide |
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