A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus |
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| Authors: | T L Barsby S A Yarrow J F Shepard |
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| Institution: | (1) Allelix Inc., 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada;(2) Present address: R. R. No. 1, LOP 1NO TerraCotta, Ontario, Canada |
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| Abstract: | Protoplasts of several spring and winter varieties of Brassica napus were isolated from hypocotyl tissue. Protoplasts divided and formed cell colonies at high frequency, without browning when cultured in modified Shepards' medium. This high efficiency of proliferation was sustained through to plant regeneration with all varieties cultured. This has been attributed to the incorporation of a reservoir medium, the presence of 2,4-D in the proliferation medium, and the presence of kinetin in conjunction with lowering of the sucrose concentration in the regeneration medium.Abbreviations NAA
1-naphthaleneacetic acid
- BAP
6-benzylaminopurine
- IAA
3-indoleacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid |
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