Effects of leptin gene expression in mice in vivo by electroporation and hydrodynamics-based gene delivery |
| |
Authors: | Xiang Lan Murai Atsushi Sugahara Kunio Yasui Akihiro Muramatsu Tatsuo |
| |
Affiliation: | Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. |
| |
Abstract: | In vivo electroporation and hydrodynamics-based gene delivery were utilized to test the effect of leptin gene transfer on food intake, and body and fat weights of mice. Gene transfer of pVRmob by electroporation caused a significant reduction in body weight compared with the control counterpart (p<0.05), although a lesser effect was found in food intake, and the weights of interscapular brown and epididymal fat by electroporation. As might be expected, the hydrodynamics-based transfection method significantly reduced body weight over 1 week post-transfection (p<0.05). Furthermore, epididymal fat was decreased by 50% at 1 week after gene transfer (p<0.001). These results suggest that both electroporation and hydrodynamics-based gene delivery may be effective approaches for systemic delivery of recombinant leptin to the central nervous system, and that the efficiency of gene transfer in hydrodynamics-based gene delivery was markedly higher than that in electroporation at least within the first week after transfection. |
| |
Keywords: | In vivo electroporation Hydrodynamics-based gene delivery Leptin expression vector Food intake Body weight |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|