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Production of cellulolytic enzymes from theXylaria andHypoxylon species of xylariaceae |
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| Authors: | D. -L. Wei S. -C. Chang Y. -H. Wei Y. -W. Lin C. -L. Chuang S. -C. Jong |
| Affiliation: | (1) Division of Biology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China;(2) Department of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China;(3) Department of Mycology, American Type Culture Collection, Parklawn Drive, 20852 Rockville, Maryland, USA |
| Abstract: | Eighteen strains of xylariaceous fungi have been screened for higher activities of cellulolytic enzymes,Trichoderma reesei QM 9414 was also examined for comparison. Strains ofXylaria anisopleura andX. regalis had higher endocellulase (CMCase) and exocellulase (Avicelase) activities after 2 weeks' incubation.Hypoxylon stygium produced the highest activity of  -glucosidase 3 days after inoculation. The optimum pH for these cellulolytic enzymes was approx. 5.0 and the optimum temperatures ranged from 37 to 50°C. A mixed culture process usingT. reesei QM 9414 andH. stygium was developed to obtain enhanced synthesis of cellulase. -Glucosidase activities in the mixed culture increased within 48h whenH. stygium was introduced after 24h. |
| Keywords: | Avicelase (exocellulase) CMCase (endocellulase) |
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