Migratory capacity and function of dendritic cells from mesenteric afferent lymph nodes after feeding a single dose of vitamin A |
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| Institution: | 1. Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco, Pernambuco, Brazil;2. Department of Tropical Medicine, Federal University of Pernambuco, Pernambuco, Brazil;3. Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Pernambuco, Brazil;4. Laboratory of Immunogenetics, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Pernambuco, Brazil;5. Laboratory of Immunoparasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Pernambuco, Brazil;6. Federal University of Pernambuco, Pernambuco, Brazil;1. Departamento de Biología Funcional, Inmunología, Facultad de Medicina, Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain;1. Vaccine and Immunotherapy Center, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA;2. Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;3. Center for Systems and Computational Biology, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA;4. Inovio Pharmaceuticals, Inc., Plymouth Meeting, PA 19462, USA;1. Blood Research Institute, Versiti, Milwaukee, WI;2. Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig University, Giessen, Germany;3. Department of Pharmacology, Medical College of Wisconsin, Milwaukee, WI;4. Department of Cell Biology, Medical College of Wisconsin, Milwaukee, WI;1. Department of Pathology, University of Michigan, Ann Arbor, MI, USA;2. Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA;3. Internal Medicine, University of Michigan, Ann Arbor, MI, USA |
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| Abstract: | Lamina propria dendritic cells (DCs) have a permanent turnover with constitutive migration to mesenteric lymph nodes and replenishment by progenitors. Luminal bacteria and dietary constituents provide key signals that endow DCs their unique properties in vivo. Taking into account that the intestinal immune system is greatly influenced by retinoids, we evaluated in B6 mice 3, 8, 16 and 24 h after feeding a single dose of vitamin A phenotype and function of cells present in mesenteric afferent lymph nodes as well as signals involved in migration. We studied the frequency of CD11c+MHC-II+CD103+CD86+ and RALDH+ DCs by flow cytometry, we determined CCL-21 and D6 levels in tissue homogenates by Western blot, and we co-cultured cells isolated from afferent lymphatics with sorted CD4+ lymphocytes to assess Foxp-3 induction and homing receptor expression. Sixteen hours after vitamin A administration, DCs isolated from afferent lymphatics were able to induce homing receptors and Foxp3 expression in CD4+ lymphocytes. Our results show that a single dose of vitamin A generated a stream of signals and amplified the tolerogenic activity of DCs migrating to lymphoid tissue. |
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