毕赤酵母细胞工厂工程化改造与应用

毕赤酵母作为细胞工厂在小分子代谢产物发酵和蛋白制品生物合成中扮演着重要角色，具有极其重要的工业应用价值。随着CRISPR/Cas9等新型编辑工具的开发和应用，对毕赤酵母细胞工厂进行多基因高效率的工程化改造已成为可能。本文首先对毕赤酵母工程化改造的遗传操作技术和目标方向进行了归纳总结，其次介绍了毕赤酵母作为细胞工厂的应用现状，同时探讨了毕赤酵母细胞工厂的优点及缺陷，并对其发展方向作出展望；以期为未来的毕赤酵母工程化改造研究提供参考和启示，推动毕赤酵母细胞工厂在生物产业中的创新应用。

Engineering and application of Komagataella phaffii as a cell factory

Nowadays, engineered Komagataella phaffii plays an important role in the biosynthesis of small molecule metabolites and protein products, showing great potential and value in industrial productions. With the development and application of new editing tools such as CRISPR/Cas9, it has become possible to engineer K. phaffii into a cell factory with high polygenic efficiency. Here, the genetic manipulation techniques and objectives for engineering K. phaffii are first summarized. Secondly, the applications of engineered K. phaffii as a cell factory are introduced. Meanwhile, the advantages as well as disadvantages of using engineered K. phaffii as a cell factory are discussed and future engineering directions are prospected. This review aims to provide a reference for further engineering K. phaffii cell factory, which is supposed to facilitate its application in bioindustry.