Dietary salecan reverts partially the metabolic gene expressions and NMR-based metabolomic profiles from high-fat-diet-induced obese rats |
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| Institution: | 1. Department of Toxicology, Shaanxi Key Lab of Free Radical Biology and Medicine, the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi’an 710032, China;2. Cardiovascular Research Institute, University of California, San Francisco, United States;3. Department of Medicine, University of California, San Francisco, United States;1. Natural Resources Institute Finland (Luke), Koetilantie 5, 00790 Helsinki, Finland;2. Natural Resources Institute Finland (Luke), Tietotie, 31600 Jokioinen, Finland;3. Biotaito, Killintie 14, 31300 Tammela, Finland;1. Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China;2. School of Material Science and Engineering, Jiangsu Engineering Centre for Flat-Panel Displays & Solid-state Lighting, Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China;1. Ningxia Key Laboratory of Vascular Injury and Repair Research, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia, 750004, China;2. Otorhinolaryngology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China;3. Cardiology, The Second People''s Hospital of Yinchuan City, Yinchuan, Ningxia, 750004, China;1. Rare Sugar Research Center, Kagawa University, Miki-cho, Kagawa 761-0795, Japan;2. Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan |
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| Abstract: | Previous studies suggest that dietary salecan (a water-soluble β-glucan) effectively reduces high-fat-diet-induced adiposity through disturbing bile-acid-promoted emulsification in mice. However, the effects of salecan on metabolic genes and metabolites involved in lipid accumulation are mostly unknown. Here, we confirmed that dietary 3% and 6% salecan for 4 weeks markedly decreased fat accumulation in liver and adipose tissue in high-fat-diet rats, displaying a decrease in mRNA levels of SREBP1-C, FAS, SCD1 and ACC1 involved in de novo lipogenesis and a reduction of levels of GPAT1, DGAT1 and DGAT2 related to triglyceride synthesis. Dietary salecan also increased the mRNA levels of PPARα and CYP7A1, which are related to fatty acid oxidation and cholesterol decomposition, respectively. In the 1H nuclear magnetic resonance metabolomic analysis, both the serum and liver metabolite profiles differed among the control groups, and the metabolic profiles of the salecan groups were shifted toward that of the low-fat-diet group. Metabolites analysis showed that salecan significantly increased hepatic glutathione and betaine levels which are related to regulation of cellular reactive oxygen species. These data demonstrate that dietary salecan not only disturbed fat digestion and absorption but also influenced lipid accumulation and metabolism in diet-induced obesity. |
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