The RNA of chicken erythrocytes |
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| Authors: | N Maclean W Madgwick |
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| Affiliation: | 1. Agricultural College, Guangdong Ocean University, Zhanjiang 524088, PR China;2. Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, PR China;1. College of Animal Science and Technology, Sichuan Agriculture University, Ya''an, Sichuan 625014, China;2. Key laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan 650223, China |
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| Abstract: | RNA was extracted from chicken erythrocytes and analysed by polyacrylamide gel electrophoresis and sucrose gradient centrifugation. 4S RNA and heavy RNA (greater than 45S) predominated and only a little ribosomal RNA was detectable. When RNA extraction and analysis followed incubation of the isolated erythrocytes with tritiated uridine, much of the label appeared in the 4S and heavier-than-45S areas. Actinomycin D at a concentration of 10 μg/ml gave substantial reduction of incorporation of both tritiated uridine and leucine into incubated erythrocytes, suggesting a continued dependence on RNA synthesis for the residual protein synthesis occurring in these cells. |
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