Separation and identification of subpicomole amounts of methotrexate polyglutamates in animal and human biopsy material |
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| Authors: | G R Krakower P A Nylen B A Kamen |
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| Affiliation: | Department of Pediatrics, Sections of Pharmacology and Toxicology, The Medical College of Wisconsin, 8701 Watertown Plank Road, Wauwatosa, Wisconsin 53226 USA |
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| Abstract: | A rapid, quantitative procedure for separating methotrexate (MTX) polyglutamates utilizing molecular-sieve column chromatography has been developed. MTX polyglutamates were separated in 25 min and detected by a radioassay with a sensitivity of 0.1 pmol MTX, allowing analysis on 2 to 5 mg of tissue. This technique has been used to demonstrate and quantitate the levels of MTX polyglutamates in liver, kidney, and brain tissues of rats treated with weekly low doses of MTX, and in liver and red cells of patients with acute leukemia treated with weekly low doses of MTX. |
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| Keywords: | To whom requests for reprint should be addressed. |
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