The Respiratory Chain of Chlorella protothecoides: II. Isolation and Characterization of Mitochondria |
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| Authors: | Grant N G Hommersand M H |
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| Institution: | Department of Botany, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514. |
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| Abstract: | Mitochondria were isolated from glucose-bleached Chlorella protothecoides Krüger, Indiana strain 25. These mitochondria oxidized succinate, NADH, and l-malate at high rates. Oxygen uptake with these substrates was partially inhibited by 1 mmm-chlorobenzhydroxamic acid (mCLAM). Respiratory control was seen with succinate as substrate in the presence of mCLAM. The apparent Km for succinate was determined to be 0.83 mm. Chlorella mitochondria catalyzed the oxidation of reduced horse heart cytochrome c. |
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