Establishment of hairy root cultures of Psoralea species |
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Authors: | Christophe Nguyen Frédéric Bourgaud Paul Forlot Armand Guckert |
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Institution: | (1) Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France;(2) Laboratoires Pharmaceutiques BERGADERM. 46, rue de la Grosse Pierre, Silic 189, 94563 Rungis Cedex, France |
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Abstract: | Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours. Whatever the culture conditions, the two furanocoumarins usually found in roots of Psoralea plants, psoralen and angelicin, were not detected in cultured transformed and non transformed roots even when some chitosan was added to the medium. However, 669 g.g–1 dry matter of psoralen and 215 g.g–1 dry matter of angelicin were found in roots from soil grown plants. A possible translocation of these compounds from the aerial parts to the roots is suggested.Abbreviations B5 medium
Gamborg's medium (Flow laboratories's formulation)
- NAA
Naphthaleneacetic acid |
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Keywords: | Agrobacterium rhizogenes angelicin furanocoumarin Psoralea psoralen transformed root |
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