|
|||||
|
|
Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity |
|
| Authors: | Masud Mohammad Mehedi Masuda Tomokazu Inoue Yusuke Kuwahara Masayasu Sawai Hiroaki Ozaki Hiroaki |
| Affiliation: | a Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho 1-5-1, Kiryu, Gunma 376-8515, Japan b Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh |
| Abstract: | Short interfering RNA (siRNA) induces specific gene silencing by the RNA interference (RNAi) pathway. Nucleosides in the 3′-overhang regions of siRNAs were replaced with 5-bis(aminoethyl)aminoethylcarbamoylmethyl-2′-deoxyuridine or thymidine. siRNA bearing modified nucleoside was more active in silencing the gene expression of hepatocyte nuclear factor 4α (HNF4α) compared with siRNA bearing thymidine. |
| Keywords: | RNAi siRNA Modified siRNA HNF4α Modified CPG support |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |