Temporal and tissue localization of a cowpea (Vigna unguiculata) cystatin |
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| Authors: | Victor M. Q. Flores,Ricardo P. Louro,José Xavier-Filho,Daniel H. P. Barratt,Peter R. Shewry, Ká tia V. S. Fernandes |
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| Affiliation: | Laboratório de Biotecnologia –LBT, Centro de Biociências e Biotecnologia, CBB, Universidade Estadual do Norte Fluminense –UENF, Campos dos Goytacazes, Rio de Janeiro –RJ, Brazil;Laboratório de Ultra-Estrutura Vegetal, Departamento de Botânica, Centro de Ciências da Saúde –CCS Universidade Federal do Rio de Janeiro –UFRJ, RJ, Brazil;Laboratório de Química e Função de Proteínas e Peptídeos –LQFPP, Centro de Biociências e Biotecnologia –CBB, Universidade Estadual do Norte Fluminense –UENF, Campos dos Goytacazes, RJ, Brazil;IACR –Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK |
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| Abstract: | A cowpea ( Vigna unguiculata L. Walpers cv. Pitiúba) cystatin was analysed to determine its localization during development and germination of cowpea seeds, using western blotting with a specific antiserum. The pattern of immunoreactive proteins changed during development, with the major reactive bands present in dried seeds being mobilized after a 62-h period of imbibition. Immunohistochemical analysis revealed that cowpea cystatin is distributed in both embryonic axes and cotyledons with the highest level being present in the outer cell walls of the adaxial surface of the cotyledons. |
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