Modification to EPA Method 1623 to address a unique seasonal matrix effect encountered in some U.S. source waters |
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| Institution: | 1. Shaw Environmental, Inc., 26 W. Martin Luther King Dr., Cincinnati, Ohio 45219, United States;2. U.S. Environmental Protection Agency, Region 10 Laboratory, 7411 Beach Dr. East, Port Orchard, WA 98366, United States;3. Analytical Services, Inc., 130 Allen Brook Lane, Williston, Vermont 05495, United States;1. Stefan S Nicolau Institute of Virology, Romanian Academy, Bucharest, Romania;2. Faculty of Chemistry, University of Bucharest, Romania;3. Institute of Cardiovascular Diseases Prof. Dr. C.C. Iliescu, Microbiology Department, Bucharest, Romania;4. University of Orleans, ICOA-UMR7311, CNRS, France;1. Area of Parasitology, Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/ Domingo Miral s/n, Zaragoza, Spain;2. Metropolitan District of Granada''s Epidemiology and Programs Unit, C/ Gustavo Dore, no. 3, Bajo, Granada, Spain;3. Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain;4. Department of Microbiology, Faculty of Medicine, University of Granada, Avenida de Madrid, s/n, Granada, Spain;1. Department of Animal and Human Physiology, School of Biology, University of Athens, University Campus, Athens 157 84, Greece;2. Department of Medicinal Chemistry, School of Pharmacy, University of Athens, Athens 157 71, Greece;1. LGCR SMRPD Chemical Research, Sanofi US, 153-1-130, 153 2nd Ave, Waltham, MA 02451, USA;2. Sanofi US, 55C-420A, 55 Corporate Drive, Bridgewater, NJ 08807, USA;3. Proteogenomics, Sanofi/Genzyme R&D Center, 49 New York Avenue, Framingham, MA 01701, USA;4. Sanofi R&D, 1, Avenue Pierre Brossolette, Chilly-Mazarin 91385, France |
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| Abstract: | U.S. Environmental Protection Agency (EPA) Method 1623 is designed to detect and determine concentrations of Cryptosporidium oocysts and Giardia cysts in water through concentration, immuno-magnetic separation (IMS), and immuno-fluorescence assay with microscopic examination. A seasonal interference with the method was observed in some municipal source waters collected from reservoirs and as reported to Shaw Environmental, Inc. in the summers of 2005, 2006, and 2007. This interference, which was not confined to a single region of the nation, caused clumping of the IMS beads during the acid dissociation of the IMS procedure in Method 1623. This effect lowered method recoveries for both Cryptosporidium and Giardia; however, the effect was more pronounced for Giardia. A heat dissociation technique (Ware et al., (2003) J. Microbiol. Methods 55, 575–583) was shown to be a viable option for samples which demonstrate the clumping matrix effect and improved Giardia recoveries in partially clumped samples. The heat dissociation application holds promise for fully clumped samples and warrants further investigation. |
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