Chemoenzymatic preparative separation of silybins A and B |
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| Affiliation: | 1. Institute of Banking and Finance, HEC - University of Lausanne, 1015 Lausanne/VD, Switzerland;2. Faculty of Economics, University of Kragujevac, Djure Pucara 3, 34000 Kragujevac, Serbia;3. Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia;4. Faculty of Economics, University of Belgrade, Kamenička 6, 11000 Beograd, Serbia;5. National Bank of Serbia, Kralja Petra 12, 11000 Beograd, Serbia;6. CESIfo, Munich, Germany;7. School of Business, Management and Economics, University of Sussex, Sussex House, Falmer, Brighton, BN1 9RH UK |
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| Abstract: | Robust and scalable chemoenzymatic procedure for obtaining gram amounts of pure silybin A and B within one week is described. It consists of the preparation of 23-O-β-pentaacetyl-galactopyranosides of silybin, their separation by silica gel chromatography, deprotection, and enzymatic sugar removal with β-galactosidase. |
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