Immobilization of maltogenase onto polyurethane microparticles from poly(vinyl alcohol) and hexamethylene diisocyanate |
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| Institution: | 1. Department of Polymer Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania;2. Laboratory of Immunoanalysis and Nanotechnology, Institute of Immunology of Vilnius University, Moletu pl. 29, 08409 Vilnius, Lithuania;3. Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania;1. Department of Physics of Polymers and Crystals, Faculty of Physics, M.V. Lomonosov Moscow State University, Leninskie Gory 1, 119991, Moscow, Russia;2. A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova St. 28, INEOS, 119991, Moscow, Russia;3. Institute of Advanced Energy-Related Nanomaterials, University of Ulm, Albert-Einstein-Allee 11, D-89069, Ulm, Germany;4. D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia;1. Universidad Central de Chile, Facultad de Ciencias de la Salud, Instituto de Investigación en Innovación en Salud, Lord Cochrane 417, Santiago, Chile;2. Universidad Andres Bello, Facultad de Ciencias Exactas, Departamento de Ciencias Químicas, Quillota 980, Viña del Mar, Chile;3. Centro para el Desarrollo de la Nanociencia y la Nanotecnología, CEDENNA, Chile;1. Depto. Física Aplicada II, Univ. del País Vasco UPV/EHU, Spain;2. Depto. Electricidad y Electrónica, Univ. del País Vasco UPV/EHU, Spain;3. BCMaterials, UPV/EHU Science Park, Spain;1. Department of Energy Science, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea;2. Department of Polymer Science and Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea;3. Department of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea;4. Information Technology & Electronic Materials Research Institute, LG Chem, Ltd. Research Park, 104-1, Moonji-dong, Yuseong-gu, Daejeon, Republic of Korea;6. Center for Biomaterials, Korea Institute of Science and Technology, 02792, Hwarang-ro, Seongbuk-gu, Seoul, Republic of Korea;1. AMME-LECAP EA4528 International Laboratory, Institut des Matériaux de Rouen, Université et INSA de Rouen, BP12, 76801 Saint Etienne du Rouvray Cedex, France;2. Institute for Chemical and Physical Processes, CNR, Largo Pontecorvo 3, 56127 Pisa, Italy;1. Department of Dyestuff Technology, Institute of Chemical Technology, N. P. Marg, Matunga, Mumbai (MH), 400019, India;2. Centre for Molecular and Biophysics Research, Department of Physics, Mar Ivanios College, Thiruananthapuram, Kerala, 695015, India |
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| Abstract: | A series of porous polyurethane (PU) microparticles from poly(vinyl alcohol) (PVA) and hexamethylene diisocyanate (HMDI) using different ratios of components were obtained by one step method. Molar compositions of PU microparticles were estimated by determination of nitrogen, isocyanate and hydroxyl groups. PU carriers which were synthesized using optimal initial molar ratios of PVA and HMDI were applied for immobilization of maltogenase (MG) from Bacillus stearothermophilus. Immobilized enzyme exhibited higher catalytic activity and enhanced temperature stability in comparison with the native MG. Maximal loading 7.78 mg/g wet carrier was reached when PU microparticles with initial molar ratio of PVA and HMDI = 1:3 was used as a carrier for immobilization. The high efficiency of immobilization (EI) was obtained using PU microparticles when initial molar ratio of HMDI and PVA was 1:1–1:10. High stability of MG immobilized onto PU microparticles during storage was demonstrated. Immobilized starch hydrolyzing enzyme was successfully tested in batch and column type reactors for hydrolysis of potato starch. MG immobilized onto PU enables easy separation from the reaction medium and reuse of the immobilized preparation over seven reaction cycles in bath operation and at least three cycles in column type reactor. |
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