The advantages of LDL (Low Density Lipoproteins) in the cryopreservation of canine semen |
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| Institution: | 1. Laboratory of Biotechnology and Reproductive Pathology, National Veterinary School of Nantes, BP 40706, 44307 Nantes, France;2. UR1268 Biopolymères Interactions Assemblages, Equipe Interfaces et Systèmes Dispersés, INRA, F-44316 Nantes Cedex 3, France;3. IMV Technologies, 10 rue Clemenceau, BP 81, 61302 Aigle Cedex, France;4. Department of Reproductive Pathology, Mother and Child, CHU Hôtel Dieux, 1 place Alexis Ricordeau-Nantes, 44093 Nantes Cedex 1, France;1. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, USA;2. Advanced Care Veterinary Hospital, Sapulpa, OK, USA;3. Kokopelli Assisted Reproductive Services, Elk Grove, CA, USA;4. Guide Dogs for the Blind, San Rafael, CA, USA;1. College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, Brazil;2. IBVET, Jaguariúna, Sao Paulo, Brazil;3. School of Veterinary Medicine, Santo Amaro University, UNISA, São Paulo, Brazil;1. Laboratory of Carnivores Reproduction, State University of Ceara, Fortaleza, Brazil;2. Faculty Earth Northeast, Caucaia, Brazil;3. Academic Unit of Serra Talhada, Federal Rural University of Pernambuco, Serra Talhada, Brazil;4. Department of Animal Science, Federal University of Ceara, Fortaleza, Brazil;1. Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna, Austria;2. Department of Animal Reproduction & Veterinary Radiology, Faculty of Veterinary Medicine, FMVZ - Sao Paulo State University UNESP, Botucatu, Brazil;3. Obstetrics, Gynecology, Andrology, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna, Austria;1. Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brasil Filho, 64, Niterói, RJ, 24320-340, Brazil;2. Faculdade de Medicina Veterinária, Universidade do Grande Rio, Rua Professor José de Souza Herdy, 1160, Duque de Caxias, RJ, 25071-202, Brazil;3. Faculdade de Medicina Veterinária, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465, Km 07, Seropédica, RJ, 23890-000, Brazil;1. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California, USA;2. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California, USA;3. Guide Dogs for the Blind, California Campus, San Rafael, California, USA;4. Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA |
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| Abstract: | A medium containing LDL (Low Density Lipoproteins, the cryoprotective component of chicken egg yolk) was compared with egg yolk for the preservation canine spermatozoa during the freeze–thaw process. Twenty sperm samples taken from 10 dogs were frozen in liquid nitrogen at −196 °C in seven different media: one control medium containing 20% egg yolk, and six test media containing 4%, 5%, 6%, 7%, 8%, and 10% LDL, respectively.Following thawing, sperm motility was assessed using a Hamilton-Thorne Sperm Analyser equipped with the CEROS 12 software. The percentage of motile spermatozoa was 55.3% in the 6% LDL medium (optimal concentration) compared with 27.7% in the egg yolk based medium (p < 0.05).In comparison with the egg-yolk medium, the LDL medium also resulted in an improved preservation of spermatozoa during the freezing process (p < 0.05) in terms of acrosomal integrity (FITC-PSA test), flagellar plasma membrane integrity (HOS test), and DNA integrity (Acridine Orange test).In addition, six Beagle bitches were inseminated twice, via the intra-uterine route, at an interval of 24 h; 200 × 106 spermatozoa that had been previously frozen in the 6% LDL medium were used per insemination. All of the bitches became pregnant (gestation rate of 100%).In conclusion, the 6% LDL medium provides improved protection of the spermatozoa during the freeze–thaw process and a marked improvement in the motility parameters of canine spermatozoa in comparison with the control medium containing egg yolk alone.Finally, the use of LDL as a cryoprotectant for canine semen does not interfere with fertility. |
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