Improvement in the in vitro maturation rate of porcine oocytes vitrified at the germinal vesicle stage by treatment with a mitochondrial permeability transition inhibitor |
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| Institution: | 1. Heart Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA;2. Department of Family Medicine, University of Oklahoma, Tulsa, Oklahoma, USA;3. Division of Cardiology, Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri, USA;1. Division of Cardiology, The Children''s Hospital of Philadelphia, Philadelphia, Pennsylvania;2. New England Research Institutes, Boston, Massachusetts;3. Department of Cardiology, Children''s Hospital, Boston, Boston, Massachusetts;4. Division of Cardiology, A.I. DuPont Hospital for Children, Wilmington, Delaware;5. Division of Cardiology, Medical College of Wisconsin, Milwaukee, Wisconsin;6. Labatt Family Heart Centre, The Hospital for Sick Children, Toronto, Ontario, Canada;7. Division of Cardiology, Johns Hopkins All Children''s Heart Institute, St. Petersburg, Florida;8. Division of Pediatric Cardiology, Duke University Medical Center, Raleigh, North Carolina;9. Division of Cardiology, Cincinnati Children''s Hospital, Cincinnati, Ohio;10. Division of Cardiology, Morgan Stanley Children''s Hospital, New York, New York;11. Division of Cardiology, Children''s Hospital Los Angeles, Los Angeles, California;12. Division of Cardiology, Primary Children''s Hospital, Salt Lake City, Utah;13. Division of Cardiac Surgery, University of Michigan Health System, Ann Arbor, Michigan;14. Division of Pediatric Cardiology, University of Michigan Health System, Ann Arbor, Michigan;15. Division of Cardiology, Texas Children''s Hospital, Houston, Texas;p. Division of Pediatric Cardiology, Medical University of South Carolina, Charleston, South Carolina;1. Department of Obstetrics and Gynecology, Yidu Central Hospital of Weifang City, China;2. Department of Obstetrics and Gynecology, Central Hospital of Qingdao City, China;3. Department of Reproductive Medicine, People''s Hospital of Laiwu City, China;4. Department of Thoracic Surgery, People''s Hospital of Qingzhou City, China;1. Department of Pediatric Cardiothoracic Surgery, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio;2. Department of Pediatric Cardiology, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio |
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| Abstract: | In this study, we examined the effects of inhibitors of mitochondrial permeability transition (MPT), caspase activity, intracellular Ca2+ chelator and mitochondrial Ca2+ uniporter on survival assessed by morphological observation and in vitro maturation (IVM) of porcine vitrified germinal vesicle (GV) oocytes. When vitrified GV oocytes were matured only present in the IVM medium with an MPT inhibitor, cyclosporin A (CsA), the survival and IVM rates (36.1% and 26.8%, respectively) were significantly higher (P < 0.05) than those in the other vitrified groups (10.3–12.3% and 6.2–10.3%, respectively). However, Z-VAD-fmk (Z-VAD), a caspase inhibitor, did not improve the survival and IVM rates (11.7–21.6% and 8.5–155%, respectively). When BAPTA-AM, an intracellular Ca2+ chelator, was present in the IVM medium, the survival and IVM rates of vitrified GV oocytes (34.5–36.2% and 25.0–26.9%, respectively) were significantly higher (P < 0.05) than those in the absent vitrified groups (17.2–24.2% and 12.9–19.3%, respectively). When ruthenium red (RR), an inhibitor of mitochondrial Ca2+ uniporter, was present only in the IVM medium, the survival and IVM rates (54.5% and 39.4%, respectively) were significantly higher than those in the other vitrified groups (25.8–38.4% and 14.4–24.2%, respectively). Furthermore, blastocysts were successfully produced using porcine vitrified GV oocytes matured in the IVM medium with RR after in vitro fertilization.These results suggested that CsA, BAPTA-AM and RR but not Z-VAD have improved the survival and IVM rates of porcine vitrified GV oocytes. |
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