A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase |
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Authors: | M Stio P Vanni G Pinzauti |
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Institution: | Dipartimento di Scienze Biochimiche, Università di Firenze, Italy. |
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Abstract: | A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corresponds to a delta E of 0.24 min-1, when the enzyme is assayed in a microsomal fraction. We also discuss the method's suitability for subcellular fractionation. No other continuous assay for this important enzymatic marker of the endoplasmic reticulum is currently available. |
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