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Improved method for transport of living cell cultures |
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| Authors: | Nikolai G Rainov Christel Trümpler Ariel Quinones Matthew A Spear Christof M Kramm |
| Institution: | 1. Department of Neurosurgery, Laboratory of Molecular Oncology, Martin-Luther-University Halle-Wittenberg, Halle, Germany 2. Gene Therapy Program, Translation Cancer Research Program, UCSD Cancer Center, Radiation Oncology, University of California at San Diego, San Diego, CA, 92103, USA 3. Department of Pediatrics, Heinrich-Heine-University, Düsseldorf, Germany |
| Abstract: | An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers during several days of storage and shipment at ambient temperature. Replating results in an identical repopulation in all cell lines. Not only tumor cells but also fibroblasts seem to tolerate well this improved method for shipment. |
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