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Catabolic potential of multiple PCB transformation systems in Rhodococcus sp. strain RHA1
Authors:Masashi Seto  Masakatsu Ida  Noriko Okita  Takashi Hatta  Eiji Masai  Masao Fukuda
Institution:(1) Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Japan;(2) Research Development Corporation of Japan, Shinsan, Nagaoka, 940-21 Niigata, Japan
Abstract:Summary Polychlorinated biphenyl (PCB) transformation activity of a strong PCB degrader, Rhodococcus sp. strain RHA1, was examined in different concentrations of PCBs. A extremely strong PCB transformation activity was observed on 30 mgrg PCB/ml. At 50 and 100 mgrg/ml, transformation activities were diminished. In the case of bphA insertion mutant, RDA1, transformation activity in the presence of ethylbezene was poor even at 30 mgrg/ml. This indicated that the bphA dependent system would play a major role in PCB transformation by RHA1. Greater transformation activity of RHA1 was observed in the presence of ethylbenzene than in the presence of biphenyl.
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